Sterilization

Ozone at ambient temperatures is the only substance which can be used as a total sterilizing agent and a substitute for high temperature. Pyrogens, byproducts of microbial growth that are toxic to humans, are not eliminated after normal autoclaving or dry heat sterilization. Pyrogens adhere firmly to surfaces of containers and are removed
only after heating at very high temperatures for extended periods of time. Because pyrogenic material is a lipopolysaccharide, the unsaturated double bonds are easily oxidized by ozone. Therefore, ozone has a distinct advantage over other depyrogenation methods.